DGAT1 抗体 (Middle Region)
Quick Overview for DGAT1 抗体 (Middle Region) (ABIN3043266)
抗原
See all DGAT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 280-318, Middle Region
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原理
- Anti-DGAT1 Antibody Picoband®
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序列
- RRILEMLFFT QLQVGLIQQW MVPTIQNSMK PFKDMDYSR
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-DGAT1 Antibody Picoband® (ABIN3043266). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human DGAT1, different from the related mouse and rat sequences by one amino acid.
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亚型
- IgG
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anti-Diacylglycerol O-Acyltransferase 1 (DGAT1) (AA 200-300) antibody
DGAT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol O-Acyltransferase 1 (DGAT1) (Internal Region) antibodyVerified DGAT1 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-Diacylglycerol O-Acyltransferase 1 (DGAT1) (AA 251-350) antibodyDGAT1 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol O-Acyltransferase 1 (DGAT1) (AA 67-79) antibodyVerified DGAT1 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Diacylglycerol O-Acyltransferase 1 (DGAT1) (AA 1-62) antibodyDGAT1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol O-Acyltransferase 1 (DGAT1) (AA 180-427) antibodyDGAT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Cases, S., Smith, S. J., Zheng, Y.-W., Myers, H. M., Lear, S. R., Sande, E., Novak, S., Collins, C., Welch, C. B., Lusis, A. J., Erickson, S. K., Farese, R. V., Jr. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Nat. Acad. Sci. 95: 13018-13023, 1998. 2. Cheng D, Iqbal J, Devenny J, Chu CH, Chen L, Dong J, Seethala R, Keim WJ, Azzara AV, Lawrence RM, Pelleymounter MA, Hussain MM (October 2008)."Acylation of acylglycerols by acyl coenzyme A:diacylglycerol acyltransferase 1 (DGAT1). Functional importance of DGAT1 in the intestinal fat absorption". J. Biol. Chem. 283 (44): 29802-11. 3. Herker, E., Harris, C., Hernandez, C., Carpentier, A., Kaehlcke, K., Rosenberg, A. R., Farese, R. V., Jr., Ott, M. Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1. Nature Med. 16: 1295-1298, 2010. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))
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别名
- DGAT1
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背景
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Synonyms: Diacylglycerol O-acyltransferase 1,2.3.1.20 ,ACAT-related gene product 1,Acyl-CoA retinol O-fatty-acyltransferase,ARAT,Retinol O-fatty-acyltransferase,2.3.1.76 ,Diglyceride acyltransferase,DGAT1,AGRP1, DGAT,
Tissue Specificity: Widely expressed.
Background: Acyl-CoA: diacylglycerol acyltransferase (DGAT) is a microsomal enzyme that plays a central role in the metabolism of cellular diacylglycerol lipids and catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT had been considered necessary for adipose tissue formation and essential for survival. There are two isozymes of DGAT encoded by the genes DGAT1 and DGAT2. DGAT1 is a host factor for HCV infection that binds core protein, localizes it to DGAT1-generated lipid droplets, and recruits viral RNA replication complexes for viral assembly. DGAT2-generated lipid droplets formed normally in cells treated with the DGAT1 inhibitor, suggesting that DGAT1 inhibitors may be useful as antiviral therapeutics.
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分子量
- 60 kDa
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基因ID
- 8694
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UniProt
- O75907
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途径
- Hormone Transport
抗原
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