DDT 抗体 (C-Term)
Quick Overview for DDT 抗体 (C-Term) (ABIN3043212)
抗原
See all DDT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 99-118, C-Term
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原理
- Anti-D-dopachrome decarboxylase DDT Antibody Picoband®
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序列
- RFFPLESWQI GKIGTVMTFL
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-D-dopachrome decarboxylase DDT Antibody (ABIN3043212). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human DDT, different from the related mouse and rat sequence by two amino acids.
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亚型
- IgG
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anti-D-Dopachrome Tautomerase (DDT) antibody
DDT 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-D-Dopachrome Tautomerase (DDT) (AA 2-118) antibodyDDT 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-D-Dopachrome Tautomerase (DDT) (AA 1-118) antibodyDDT 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G1 unconjugated
anti-D-Dopachrome Tautomerase (DDT) antibodyDDT 适用: 人 WB, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-D-Dopachrome Tautomerase (DDT) antibodyDDT 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-D-Dopachrome Tautomerase (DDT) (AA 50-90) antibodyDDT 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-D-Dopachrome Tautomerase (DDT) (AA 2-118) antibodyDDT 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-D-Dopachrome Tautomerase (DDT) (AA 1-118) antibodyDDT 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-D-Dopachrome Tautomerase (DDT) (C-Term) antibodyDDT 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-D-Dopachrome Tautomerase (DDT) (AA 1-118) antibodyDDT 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
1. Esumi, N., Budarf, M., Ciccarelli, L., Sellinger, B., Kozak, C. A., Wistow, G.Conserved gene structure and genomic linkage for D-dopachrome tautomerase (DDT) and MIF.Mammalian Genome 9: 753-757, 1998. 2. Nishihira, J., Fujinaga, M., Kuriyama, T., Suzuki, M., Sugimoto, H., Nakagawa, A., Tanaka, I., Sakai, M.Molecular cloning of human D-dopachrome tautomerase cDNA: N-terminal proline is essential for enzyme activation.Biochem. Biophys. Res. Commun. 243: 538-544, 1998. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- DDT (D-Dopachrome Tautomerase (DDT))
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别名
- DDT
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背景
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Synonyms: D-dopachrome decarboxylase,4.1.1.84,D-dopachrome tautomerase,Phenylpyruvate tautomerase II,DDT,
Tissue Specificity: Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
Sequence Similarities: Belongs to the intercrine alpha (chemokine CxC) family.
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分子量
- 13-15 kDa
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UniProt
- P30046
抗原
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