NOXA1 抗体 (Middle Region)
Quick Overview for NOXA1 抗体 (Middle Region) (ABIN3042993)
抗原
See all NOXA1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 176-195, Middle Region
-
原理
- Anti-NOXA1 Antibody Picoband®
-
序列
- RQVPRGEVFR PHRWHLKHLE
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-NOXA1 Antibody (ABIN3042993). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human NOXA1.
-
亚型
- IgG
-
-
anti-NADPH Oxidase Activator 1 (NOXA1) (AA 150-313) antibody
NOXA1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Activator 1 (NOXA1) (AA 1-483) antibodyNOXA1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Activator 1 (NOXA1) (AA 301-400) antibodyNOXA1 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase Activator 1 (NOXA1) (AA 1-483) antibodyNOXA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NADPH Oxidase Activator 1 (NOXA1) (AA 140-300) antibodyNOXA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human
1. Banfi, B., Clark, R. A., Steger, K., Krause, K.-H. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J. Biol. Chem. 278: 3510-3513, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/25/2007. 3. Takeya, R., Ueno, N., Kami, K., Taura, M., Kohjima, M., Izaki, T., Nunoi, H., Sumimoto, H. Novel human homologues of p47(phox) and p67(phox) participate in activation of superoxide-producing NADPH oxidases. J. Biol. Chem. 278: 25234-25246, 2003. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
-
储存液
- Thimerosal (Merthiolate), Sodium azide
-
注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
-
-
- NOXA1 (NADPH Oxidase Activator 1 (NOXA1))
-
别名
- NOXA1
-
背景
-
Synonyms: NADPH oxidase activator 1,NOX activator 1,Antigen NY-CO-31,NCF2-like protein,P67phox-like factor,p51-nox,NOXA1,P51NOX,
Tissue Specificity: Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. .
Background: NOXA1 (NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz (2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence (build 36.1). Banfi et al. (2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al. (2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX (CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
Sequence Similarities: Belongs to the NCF2/NOXA1 family.
-
分子量
- 60 kDa
抗原
-
