Cullin 3 抗体 (Middle Region)
Quick Overview for Cullin 3 抗体 (Middle Region) (ABIN3042989)
抗原
See all Cullin 3 (CUL3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 426-444, Middle Region
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原理
- Anti-Cullin 3/CUL3 Antibody Picoband®
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序列
- DVFERYYKQH LARRLLTNK
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Cullin 3/CUL3 Antibody (ABIN3042989). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Cullin 3, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Cullin 3 (CUL3) antibody
CUL3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Cullin 3 (CUL3) (AA 411-510) antibodyCUL3 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cullin 3 (CUL3) antibodyCUL3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 3 (CUL3) antibodyCUL3 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 7B6 unconjugated Recombinant Antibody
anti-Cullin 3 (CUL3) (AA 618-667) antibodyCUL3 适用: 人, 斑马鱼, Cow, 小鸡, 犬, 豚鼠, 马, 兔, 猴, Bat, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cullin 3 (CUL3) (AA 301-400) antibodyCUL3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Cullin 3 (CUL3) (N-Term) antibodyCUL3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 3 (CUL3) antibodyCUL3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cullin 3 (CUL3) (Internal Region) antibodyVerified CUL3 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Cullin 3 (CUL3) (N-Term) antibodyCUL3 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kigoshi, Y., Tsuruta, F., Chiba, T. Ubiquitin ligase activity of Cul3-KLHL7 protein is attenuated by autosomal dominant retinitis pigmentosa causative mutation. J. Biol. Chem. 286: 33613-33621, 2011. 2. Kipreos, E. T., Lander, L. E., Wing, J. P., He, W. W., Hedgecock, E. M. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85: 829-839, 1996. 3. Rondou, P., Haegeman, G., Vanhoenacker, P., Van Craenenbroeck, K. BTB protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase. J. Biol. Chem. 283: 11083-11096, 2008. 4. Sumara, I., Quadroni, M., Frei, C., Olma, M. H., Sumara, G., Ricci, R., Peter, M. A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells. Dev. Cell 12: 887-900, 2007. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- Cullin 3 (CUL3)
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别名
- CUL3
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背景
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Synonyms: Cullin-3,CUL-3,CUL3,KIAA0617,
Tissue Specificity: Widely expressed.
Background: Cullin-3 (Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al. (1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37). Sumara et al. (2007) found that KLHL9, KLHL13, and CUL3 interacted ly in a 370-kD protein complex in HeLa cell lysates. Rondou et al. (2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex ed ubiquitination of dopamine receptor D4 (DRD4). KLHL12 interacted ly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al. (2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).
Sequence Similarities: Belongs to the cullin family.
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分子量
- 89 kDa
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UniProt
- Q13618
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途径
- M Phase
抗原
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