Flotillin 1 抗体 (Middle Region)
(1 reference)-
- 抗原 See all Flotillin 1 (FLOT1) 抗体
- Flotillin 1 (FLOT1)
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抗原表位
- AA 219-234, Middle Region
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Flotillin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- Rabbit IgG polyclonal antibody for Flotillin-1(FLOT1) detection. Tested with WB in Human,Mouse,Rat.
- 序列
- KKAAYDIEVN TRRAQA
- 交叉反应 （详细）
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Flotillin-1(FLOT1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: flotillin 1
Protein Name: Flotillin-1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1(219-234aa KKAAYDIEVNTRRAQA), different from the related rat and mouse sequences by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product FLOT1 Primary Antibody
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anti-Flotillin 1 (FLOT1) (C-Term) antibody
Verified FLOT1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 169-251) antibodyFLOT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 128-427) antibodyFLOT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (C-Term) antibodyKO Validated FLOT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 101-200) antibodyFLOT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 1-427) antibodyFLOT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) antibodyFLOT1 适用: 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 6C10 unconjugated
anti-Flotillin 1 (FLOT1) (C-Term) antibody (Biotin)Verified FLOT1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal Biotin
anti-Flotillin 1 (FLOT1) (AA 104-133), (N-Term) antibodyFLOT1 适用: 人 WB 宿主: 兔 Polyclonal RB40153 unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for FLOT1 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).
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: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).
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- 抗原
- Flotillin 1 (FLOT1)
- 别名
- FLOT1 (FLOT1 产品)
- 背景
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FLOT1(Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
Synonyms: FLOT 1 antibody|FLOT1 antibody|FLOT1_HUMAN antibody|Flotillin-1 antibody|Flotillin1 antibody|Integral membrane component of caveolae antibody|Reggie 2 antibody - UniProt
- O75955
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