Flotillin 1 抗体 (Middle Region)
(1 reference)-
- 抗原 See all Flotillin 1 (FLOT1) 抗体
- Flotillin 1 (FLOT1)
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抗原表位
- AA 219-234, Middle Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Flotillin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 原理
- Anti-Flotillin 1/FLOT1 Antibody Picoband®
- 序列
- KKAAYDIEVN TRRAQA
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-Flotillin 1/FLOT1 Antibody (ABIN3042941). Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1, different from the related rat and mouse sequences by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product FLOT1 Primary Antibody
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anti-Flotillin 1 (FLOT1) (C-Term) antibody
Verified FLOT1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 169-251) antibodyFLOT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 128-427) antibodyFLOT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (C-Term) antibodyKO Validated FLOT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 101-200) antibodyFLOT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) (AA 1-427) antibodyFLOT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Flotillin 1 (FLOT1) antibodyFLOT1 适用: 小鼠, 大鼠 WB 宿主: 小鼠 Monoclonal 6C10 unconjugated
anti-Flotillin 1 (FLOT1) (C-Term) antibody (Biotin)Verified FLOT1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal Biotin
anti-Flotillin 1 (FLOT1) (AA 104-133), (N-Term) antibodyFLOT1 适用: 人 WB 宿主: 兔 Polyclonal RB40153 unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. 2. Hazarika, P., Dham, N., Patel, P., Cho, M., Weidner, D., Goldsmith, L., Duvic, M. Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. J. Cell. Biochem. 75: 147-159, 1999. 3. Santamaria, A., Castellanos, E., Gomez, V., Benedit, P., Renau-Piqueras, J., Morote, J., Reventos, J., Thomson, T. M., Paciucci, R. PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts. Molec. Cell. Biol. 25: 1900-1911, 2005. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. - 有效期
- 12 months
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: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).
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: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).
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- 抗原
- Flotillin 1 (FLOT1)
- 别名
- FLOT1 (FLOT1 产品)
- 背景
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Synonyms: Flotillin-1,FLOT1,
Tissue Specificity: Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .
Background: FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
Sequence Similarities: Belongs to the eIF-3 subunit B family.
- 分子量
- 47 kDa
- UniProt
- O75955
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