SPARCL1 抗体 (C-Term)
Quick Overview for SPARCL1 抗体 (C-Term) (ABIN3042909)
抗原
See all SPARCL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 649-664, C-Term
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原理
- Anti-SPARC-like protein 1 SPARCL1 Antibody Picoband®
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序列
- HCFGIKEEDI DENLLF
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-SPARC-like protein 1 SPARCL1 Antibody (ABIN3042909). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-SPARC-Like 1 (Hevin) (SPARCL1) antibody
SPARCL1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 17-300) antibodySPARCL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 298-545) antibodySPARCL1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 208-236) antibodySPARCL1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB24552 unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 215-244), (Middle Region) antibodySPARCL1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Girard, J.-P., Springer, T. A. Cloning from purified high endothelial venule cells of hevin, a close relative of the antiadhesive extracellular matrix protein SPARC. Immunity 2: 113-123, 1995. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))
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别名
- SPARCL1
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背景
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Synonyms: SPARC-like protein 1,High endothelial venule protein,Hevin,MAST 9,SPARCL1,
Tissue Specificity: Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
Background: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration (Girard and Springer, 1995).
Sequence Similarities: Belongs to the SPARC family.
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分子量
- 95 kDa
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UniProt
- Q14515
抗原
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