(2 references)-
- 抗原 See all E2F3 抗体
- E2F3
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抗原表位
- AA 446-465, C-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This E2F3 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- Anti-Transcription factor E2F3 E2F3 Antibody Picoband®
- 序列
- DLFDAYDLEK LPLVEDFMCS
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-Transcription factor E2F3 E2F3 Antibody (ABIN3042877). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3, identical to the related mouse sequence.
- 亚型
- IgG
- Top Product
- Discover our top product E2F3 Primary Antibody
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anti-E2F3 (AA 336-425) antibody
适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 5F7 unconjugated
anti-E2F3 (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 381-465) antibody适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (acLys168) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 1-108) antibody适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 1-133) antibody适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-E2F3 (AA 1-133) antibody适用: 人 ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-E2F3 (C-Term) antibodyVerified 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-E2F3 (AA 200-300) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. - 有效期
- 12 months
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: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, (2010) (PubMed).
: "Regulating A549 cells growth by ASO inhibiting miRNA expression." in: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, (2010) (PubMed).
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: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." in: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, (2010) (PubMed).
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- 抗原
- E2F3
- Abstract
- E2F3 产品
- 背景
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Synonyms: Transcription factor E2F3,E2F-3,E2F3,KIAA0075,
Background: Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts ly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.
Sequence Similarities: Belongs to the E2F/DP family.
- 分子量
- 49 kDa
- UniProt
- O00716
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