KLF4 抗体 (N-Term)
Quick Overview for KLF4 抗体 (N-Term) (ABIN3042832)
抗原
See all KLF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 93-108, N-Term
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原理
- Anti-Krueppel-like factor 4 KLF4 Antibody Picoband®
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序列
- RRETEEFNDL LDLDFI
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-Krueppel-like factor 4 KLF4 Antibody (ABIN3042832). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4, identical to the related mouse and rat sequences.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Moore, D. L., Blackmore, M. G., Hu, Y., Kaestner, K. H., Bixby, J. L., Lemmon, V. P., Goldberg, J. L. KLF family members regulate intrinsic axon regeneration ability. Science 326: 298-301, 2009. 2. Patel, S., Xi, Z. F., Seo, E. Y., McGaughey, D., Segre, J. A. Klf4 and corticosteroids activate an overlapping set of transcriptional targets to accelerate in utero epidermal barrier acquisition. Proc. Nat. Acad. Sci. 103: 18668-18673, 2006. 3. Wang, H., Yang, L., Jamaluddin, M. S., Boyd, D. D. The Kruppel-like KLF4 transcription factor, a novel regulator of urokinase receptor expression, drives synthesis of this binding site in colonic crypt luminal surface epithelial cells. J. Biol. Chem. 279: 22674-22683, 2004. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901." in: Stem cells international, Vol. 2016, pp. 1792573, (2016) (PubMed).
: "CHIR99021 enhances Klf4 Expression through β-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150936, (2016) (PubMed).
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: "Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901." in: Stem cells international, Vol. 2016, pp. 1792573, (2016) (PubMed).
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- KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))
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别名
- KLF4
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背景
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Synonyms: Krueppel-like factor 4,Epithelial zinc finger protein EZF,Gut-enriched krueppel-like factor,KLF4,EZF, GKLF,
Background: Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Sequence Similarities: Belongs to the krueppel C2H2-type zinc-finger protein family.
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分子量
- 47 kDa
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UniProt
- O43474
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途径
- Peptide Hormone Metabolism, Stem Cell Maintenance
抗原
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