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Cofilin 抗体 (AA 2-166)

CFL1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3042747
发货至: 中国
  • 抗原 See all Cofilin (CFL1) 抗体
    Cofilin (CFL1) (Cofilin 1 (CFL1))
    抗原表位
    • 16
    • 15
    • 14
    • 11
    • 8
    • 7
    • 7
    • 7
    • 5
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-166
    适用
    • 112
    • 74
    • 71
    • 6
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, 猴
    宿主
    • 117
    • 7
    • 2
    克隆类型
    • 116
    • 9
    多克隆
    标记
    • 62
    • 9
    • 9
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cofilin antibody is un-conjugated
    应用范围
    • 63
    • 54
    • 44
    • 31
    • 30
    • 28
    • 28
    • 19
    • 18
    • 8
    • 7
    • 6
    • 3
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    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    原理
    Anti-Cofilin/CFL1 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-Cofilin/CFL1 Antibody Picoband® (ABIN3042747). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
    亚型
    IgG
    Top Product
    Discover our top product CFL1 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000). "Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents". Cell Motil. Cytoskeleton 45 (4): 293-306. 3. Lappalainen P, Drubin DG (July 1997). "Cofilin promotes rapid actin filament turnover in vivo".Nature 388 (6637): 78-82.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    Cofilin (CFL1) (Cofilin 1 (CFL1))
    别名
    CFL1 (CFL1 产品)
    背景

    Synonyms: Cofilin-1,18 kDa phosphoprotein,p18,Cofilin, non-muscle isoform,CFL1,CFL,

    Tissue Specificity: Widely distributed in various tissues.

    Background: Cofilin, a member of the ADF/cofilin family, is actually a protein with 70 % sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH , phosphorylation and phosphoinositides regulate cofilin's binding and associating activity with actin.

    Sequence Similarities: Belongs to the actin-binding proteins ADF family.

    分子量
    19 kDa
    基因ID
    1072
    UniProt
    P23528
    途径
    Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
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