Integrin beta 4 抗体 (AA 28-266)
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- 抗原 See all Integrin beta 4 (ITGB4) 抗体
- Integrin beta 4 (ITGB4)
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抗原表位
- AA 28-266
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Integrin beta 4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- 原理
- Anti-Integrin beta 4/ITGB4 Antibody Picoband®
- 交叉反应 （详细）
- No cross-reactivity with other proteins
- 产品特性
- Anti-Integrin beta 4/ITGB4 Antibody Picoband® (ABIN3042525). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human Integrin beta 4 recombinant protein (Position: N28-A266). Human Integrin beta 4 shares 88% and 86% amino acid (aa) sequences identity with mouse and rat Integrin beta 4, respectively.
- 亚型
- IgG
- Top Product
- Discover our top product ITGB4 Primary Antibody
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anti-Integrin beta 4 (ITGB4) (AA 29-206) antibody
ITGB4 适用: 人 IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1D6B4 unconjugated
anti-Integrin beta 4 (ITGB4) (AA 29-206) antibodyITGB4 适用: 人 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5G3G5 unconjugated
anti-Integrin beta 4 (ITGB4) (AA 1401-1533) antibodyITGB4 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 4 (ITGB4) (C-Term) antibodyITGB4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 4 (ITGB4) (Tyr1510) antibodyITGB4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 4 (ITGB4) (AA 28-137) antibodyITGB4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Integrin beta 4 (ITGB4) antibodyITGB4 适用: 人, 雪貂, Mustelid WB, FACS, IP, IF, IHC (fro) 宿主: 小鼠 Monoclonal 450-9D unconjugated
anti-Integrin beta 4 (ITGB4) (AA 1098-1356) antibodyITGB4 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 4 (ITGB4) (Tyr1510) antibodyITGB4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 4 (ITGB4) (pTyr1494) antibodyITGB4 适用: 大鼠 ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Carmi, R., Sofer, S., Karplus, M., Ben-Yakar, Y., Mahler, D., Zirkin, H., Bar-Ziv, J. Aplasia cutis congenita in two sibs discordant for pyloric atresia. Am. J. Med. Genet. 11: 319-328, 1982. 2. Dajee, M., Lazarov, M., Zhang, J. Y., Cai, T., Green, C. L., Russell, A. J., Marinkovich, M. P., Tao, S., Lin, Q., Kubo, Y., Khavari, P. A. NF-kappa-B blockade and oncogenic Ras trigger invasive human epidermal neoplasia. Nature 421: 639-643, 2003. 3. Geerts, D., Fontao, L., Nievers, M. G., Schaapveld, R. Q. J., Purkis, P. E., Wheeler, G. N., Lane, E. B., Leigh, I.M., Sonnenberg, A.Binding of integrin alpha-6-beta-4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding. J. Cell Biol. 147: 417-434, 1999. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- Integrin beta 4 (ITGB4)
- 别名
- ITGB4 (ITGB4 产品)
- 背景
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Synonyms: Integrin beta-4,GP150,CD104,ITGB4,
Tissue Specificity: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Background: ITGB4 (Integrin, beta-4), also known as CD104 (Cluster of Differentiation 104), is a human gene. The gene encodes the integrin beta 4 subunits, a receptor for the laminins. This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. The ITGB4 gene is mapped on 17q25.1. Using expression profiling, Yang et al. found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells. They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines. The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III (FNIII) repeats, residing in 2 pairs separated by a connecting segment. Vidal et al. found compound heterozygosity for mutations in the ITGB4 gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia.
Sequence Similarities: Belongs to the integrin beta chain family.
- 分子量
- 210 kDa
- UniProt
- P16144
- 途径
- Integrin Complex
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