NPM1 抗体 (AA 1-294)
Quick Overview for NPM1 抗体 (AA 1-294) (ABIN3042497)
抗原
See all NPM1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1-294
-
原理
- Anti-Nucleophosmin/NPM1 Antibody Picoband®
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-Nucleophosmin/NPM1 Antibody Picoband® (ABIN3042497). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E.coli-derived human Nucleophosmin recombinant protein (Position: M1-L294). Human Nucleophosmin shares 95% amino acid (aa) sequence identity with both mouse and rat Nucleophosmin.
-
亚型
- IgG
-
-
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 1-294) antibody
NPM1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B2 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibodyNPM1 适用: 人 WB, IF, IHC (p), IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 20-154) antibodyNPM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 212-292) antibodyNPM1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal NPM1-3286 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pThr199) antibodyNPM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) antibodyVerified NPM1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rNPM1-1901 unconjugated Recombinant Antibody
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal NPM1-1902 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 198-226) antibodyNPM1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Chan, W.-Y., Liu, Q.-R., Borjigin, J., Busch, H., Rennert, O. M., Tease, L. A., Chan, P.-K. Characterization of the cDNA encoding human nucleophosmin and studies of its role in normal and abnormal growth. Biochemistry 28: 1033-1039, 1989. 2. Gale, R. E., Green, C., Allen, C., Mead, A. J., Burnett, A. K., Hills, R. K., Linch, D. C. The impact of FLT3 tandem duplication mutant level, number, size, and interaction with NPM1 mutations in a large cohort of young adult patients with acute myeloid leukemia. Blood 111: 2776-2784, 2008. 3. Zhang, Q., Wang, H. Y., Liu, X., Wasik, M. A. STAT5A is epigenetically silenced by the tyrosine kinase NPM1-ALK and acts as a tumor suppressor by reciprocally inhibiting NPM1-ALK expression. Nature Med. 13: 1341-1348, 2007. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
-
别名
- NPM1
-
背景
-
Synonyms: Nucleophosmin,NPM,Nucleolar phosphoprotein B23,Nucleolar protein NO38,Numatrin,NPM1,NPM,
Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.
Background: NPM1 (Nucleophosmin/Nucleoplasmin family, member1), also known as NPM, nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. The NPM1 gene maps to chromosome 5q35. Chan et al. (1989) found that nucleophosmin is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in nucleophosmin protein level. They stated that nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.
Sequence Similarities: Belongs to the nucleoplasmin family.
-
分子量
- 39 kDa
-
基因ID
- 4869
-
UniProt
- P06748
-
途径
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
-
