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NPM1 抗体 (AA 1-294)

This 兔 多克隆 anti-NPM1 antibody specifically detects NPM1 in WB, IHC, IF, FACS 和 ICC. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN3042497
发货至: 中国
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Quick Overview for NPM1 抗体 (AA 1-294) (ABIN3042497)

抗原

See all NPM1 抗体
NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This NPM1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
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    AA 1-294

    原理

    Anti-Nucleophosmin/NPM1 Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-Nucleophosmin/NPM1 Antibody (ABIN3042497). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E.coli-derived human Nucleophosmin recombinant protein (Position: M1-L294). Human Nucleophosmin shares 95% amino acid (aa) sequence identity with both mouse and rat Nucleophosmin.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

    别名

    NPM1

    背景

    Synonyms: Nucleophosmin,NPM,Nucleolar phosphoprotein B23,Nucleolar protein NO38,Numatrin,NPM1,NPM,

    Tissue Specificity: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.

    Background: NPM1 (Nucleophosmin/Nucleoplasmin family, member1), also known as NPM, nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. The NPM1 gene maps to chromosome 5q35. Chan et al. (1989) found that nucleophosmin is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in nucleophosmin protein level. They stated that nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.

    Sequence Similarities: Belongs to the nucleoplasmin family.

    分子量

    39 kDa

    基因ID

    4869

    UniProt

    P06748

    途径

    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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