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CD43 抗体 (AA 272-400)

SPN 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3042491
发货至: 中国
  • 抗原 See all CD43 (SPN) 抗体
    CD43 (SPN) (Sialophorin (SPN))
    抗原表位
    • 17
    • 13
    • 12
    • 10
    • 9
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 272-400
    适用
    人, 小鼠, 大鼠
    宿主
    • 95
    • 68
    • 3
    克隆类型
    • 84
    • 81
    • 1
    多克隆
    标记
    • 61
    • 18
    • 18
    • 16
    • 12
    • 10
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD43 antibody is un-conjugated
    应用范围
    • 102
    • 76
    • 62
    • 62
    • 40
    • 17
    • 17
    • 13
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    原理
    Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for Leukosialin(SPN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
    Gene Name: sialophorin
    Protein Name: Leukosialin
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.
    亚型
    IgG
    Top Product
    Discover our top product SPN Primary Antibody
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD43 is approximately 0.25 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • 抗原
    CD43 (SPN) (Sialophorin (SPN))
    别名
    SPN (SPN 产品)
    别名
    CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
    背景
    CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

    Synonyms: CD 43 antibody|CD43 antibody|CD43 antigen antibody|Galactoglycoprotein antibody|Galactoglycoprotein antibody|GALGP antibody|GPL 115 antibody|GPL115 antibody|Human gene for sialophorin antibody|Leucocyte sialoglycoprotein antibody|Leucocyte sialoglycoprotein antibody|LEUK_HUMAN antibody|Leukocyte sialoglycoprotein antibody|Leukosialin antibody|LSN antibody|Sialophorin (leukosialin) antibody|Sialophorin antibody|SPN antibody
    基因ID
    6693
    UniProt
    P16150
    途径
    Regulation of Leukocyte Mediated Immunity
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