HPSE 抗体 (Middle Region)
(3 references)Quick Overview for HPSE 抗体 (Middle Region) (ABIN3042457)
抗原
See all HPSE 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 301-331, Middle Region
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原理
- Anti-Heparanase 1/HPSE Antibody Picoband®
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序列
- NGRTATKEDF LNPDVLDIFI SSVQKVFQVV E
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Heparanase 1/HPSE Antibody Picoband® (ABIN3042457). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Heparanase 1, different from the related mouse and rat sequences by eight amino acids.
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亚型
- IgG
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anti-Heparanase (HPSE) (AA 34-115) antibody
HPSE 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 353-464) antibodyHPSE 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 40-188) antibodyHPSE 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 280-412) antibodyHPSE 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 400-543) antibodyHPSE 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Heparanase (HPSE) (AA 68-117) antibodyHPSE 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) antibodyHPSE 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 36-275) antibodyHPSE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 263-523) antibodyHPSE 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Heparanase (HPSE) (AA 72-170) antibodyHPSE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D7 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Hulett MD, Freeman C, Hamdorf BJ, Baker RT, Harris MJ, Parish CR (July 1999), "Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis", Nature medicine 5 (7): 803-9. 2. Nakajima M, Irimura T, Nicolson GL. (1988), "Heparanases and tumor metastasis", J. Cell. Biochem. 36 (2): 157-167. 3. Toyoshima, M., Nakajima, M. : Human heparanase: purification, characterization, cloning, and expression. J. Biol. Chem. 274: 24153-24160, 1999. 4. Vlodavsky I, Friedmann Y, Elkin M, Aingorn H, Atzmon R, Ishai-Michaeli R, Bitan M, Pappo O, Peretz T, Michal I, Spector L, Pecker I (July 1999), "Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis", Nature medicine 5 (7): 793-802. 5. Vlodavsky I, Goldshmidt O, Zcharia E, et al. (2003), "Mammalian heparanase: involvement in cancer metastasis, angiogenesis and normal development.", Semin. Cancer Biol. 12 (2): 121-9. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Expression and correlation of matrix metalloproteinase-9 and heparanase in patients with breast cancer." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 26, (2014) (PubMed).
: "Inhibition of choriocarcinoma by Fe3O4-dextran-anti-β-human chorionic gonadotropin nanoparticles containing antisense oligodeoxynucleotide of heparanase." in: International journal of nanomedicine, Vol. 8, pp. 4371-8, (2014) (PubMed).
: "The expression and clinical significance of microRNA-1258 and heparanase in human breast cancer." in: Clinical biochemistry, Vol. 46, Issue 10-11, pp. 926-32, (2013) (PubMed).
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: "Expression and correlation of matrix metalloproteinase-9 and heparanase in patients with breast cancer." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 26, (2014) (PubMed).
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- HPSE (Heparanase (HPSE))
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别名
- HPSE
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背景
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Synonyms: Heparanase,3.2.1.166,Endo-glucoronidase,Heparanase-1,Hpa1,Heparanase 8 kDa subunit,Heparanase 50 kDa subunit,HPSE,HEP, HPA, HPA1, HPR1, HPSE1, HSE1,
Tissue Specificity: Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. Also expressed in hair follicles, specifically in both Henle's and Huxley's layers of inner the root sheath (IRS) at anagen phase. .
Background: Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.
Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.
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分子量
- 61 kDa
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基因ID
- 10855
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UniProt
- Q9Y251
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途径
- Glycosaminoglycan Metabolic Process
抗原
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