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HMGB3 抗体 (N-Term)

This anti-HMGB3 antibody is a 兔 多克隆 antibody detecting HMGB3 in WB, IHC, ICC, IF 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN3042447
发货至: 中国

Quick Overview for HMGB3 抗体 (N-Term) (ABIN3042447)

抗原

See all HMGB3 抗体
HMGB3 (High Mobility Group Box 3 (HMGB3))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This HMGB3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 抗原表位

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    AA 62-95, N-Term

    原理

    Anti-HMG4/HMGB3 Antibody Picoband®

    序列

    EMAKADKVRY DREMKDYGPA KGGKKKKDPN APKR

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-HMG4/HMGB3 Antibody Picoband® (ABIN3042447). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4, identical to the related mouse and rat sequences.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. "Entrez Gene: HMGB3 high-mobility group box 3". 2. Davis DL, Burch JB (1992). "Isolation of a chicken HMG2 cDNA clone and evidence for an HMG2-specific 3'-untranslated region.". Gene 113 (2): 251-6. 3. Vaccari T, Beltrame M, Ferrari S, Bianchi ME (Aug 1998). "Hmg4, a new member of the Hmg1/2 gene family".Genomics 49 (2): 247-52.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    HMGB3 (High Mobility Group Box 3 (HMGB3))

    别名

    HMGB3

    背景

    Synonyms: High mobility group protein B3,High mobility group protein 2a,HMG-2a,High mobility group protein 4,HMG-4,HMGB3,HMG2A, HMG4,

    Tissue Specificity: Expressed predominantly in placenta.

    Background: High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.

    Sequence Similarities: Contains 1 HMG box DNA-binding domain.

    分子量

    23 kDa

    基因ID

    3149

    UniProt

    O15347
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