HINT1 抗体 (Middle Region)
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北京 101111
Quick Overview for HINT1 抗体 (Middle Region) (ABIN3042445)
抗原
See all HINT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-HINT1 Antibody
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序列
- HISQISVAED DDESLLGHLM IVGKKCAADL GLNK
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross-reactivity with other proteins
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预测反应
- different from the related mouse,rat sequences by three amino acids.
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产品特性
- Anti-HINT1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human HINT1, different from the related mouse and rat sequences by three amino acids.
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亚型
- IgG
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))
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别名
- HINT1
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背景
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Background: Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/β-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.
Gene Full Name: histidine triad nucleotide binding protein 1
Sequence Similarities: Contains 1 HMG box DNA-binding domain.
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分子量
- 14 kDa
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基因ID
- 3094
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UniProt
- P49773
抗原
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