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HINT1 抗体 (Middle Region)

This 兔 多克隆 antibody specifically detects HINT1 in WB, IHC, IF, ICC 和 FACS. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN3042445
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for HINT1 抗体 (Middle Region) (ABIN3042445)

抗原

See all HINT1 抗体
HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

适用

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  • 36
  • 4
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  • 2
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  • 1
人, 小鼠, 大鼠

宿主

  • 61
  • 6

克隆类型

  • 49
  • 18
多克隆

标记

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  • 1
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This HINT1 antibody is un-conjugated

应用范围

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  • 23
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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • 抗原表位

    • 15
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
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    Middle Region

    原理

    Anti-HINT1 Antibody

    序列

    HISQISVAED DDESLLGHLM IVGKKCAADL GLNK

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins

    预测反应

    different from the related mouse,rat sequences by three amino acids.

    产品特性

    Anti-HINT1 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence in the middle region of human HINT1, different from the related mouse and rat sequences by three amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    HINT1 (Histidine Triad Nucleotide Binding Protein 1 (HINT1))

    别名

    HINT1

    背景

    Background: Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene. It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/β-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells. The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. Several transcript variants, but only one of them protein-coding, have been found for this gene.

    Gene Full Name: histidine triad nucleotide binding protein 1

    Sequence Similarities: Contains 1 HMG box DNA-binding domain.

    分子量

    14 kDa

    基因ID

    3094

    UniProt

    P49773
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