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GAD 抗体 (AA 14-122)

Name: GAD 抗体 (AA 14-122)
SKU: ABIN3042415
Availability: OutOfStock

GAD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 14-122) antibody (ABIN3042415) (GAD 抗体 (AA 14-122))

Anti- GAD67 Picoband antibody, Western blotting All lanes: Anti GAD67 at 0. (GAD 抗体 (AA 14-122))

3 images

(1 reference)

产品编号 ABIN3042415

发货至: 中国

Datasheet as PDF

抗原 See all GAD (GAD1) 抗体

GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
抗原表位
14

11

8

8

8

7

4

2

2

1

1

1

1

1

1

1

1
AA 14-122
适用
92

61

53

5

4

3

3

2

2

1

1

1
人, 小鼠, 大鼠
宿主
85

15

3

2
兔
Compatible Secondaries
克隆类型
83

22
多克隆
标记
55

8

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This GAD antibody is un-conjugated
应用范围
73

33

23

18

13

13

12

12

9

7

5

4

3

2

1

1

1

1

1

1
Western Blotting (WB)

原理

Anti-GAD67/GAD1 Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-GAD67/GAD1 Antibody Picoband® (ABIN3042415). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.

亚型

IgG

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GAD1 适用: 人 WB, IHC, ICC, IHC (p), IF, IHC (fro), IHC (ff), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-97) antibody
GAD1 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 455-594) antibody
GAD1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibody
GAD1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal GAD1-2391 unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (Internal Region) antibody
Verified GAD1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 72-135) antibody
GAD1 适用: 人 WB, ELISA, FACS, Coat 宿主: 小鼠 Monoclonal GAD1-2563 unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody
GAD1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-200) antibody
GAD1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kelly, C. D., Edwards, Y., Johnstone, A. P., Harfst, E., Nogradi, A., Nussey, S. S., Povey, S., Carter, N. D. : Nucleotide sequence and chromosomal assignment of a cDNA encoding the large isoform of human glutamate decarboxylase. Ann. Hum. Genet. 56: 255-265, 1992. 2. Bu, D.-F., Erlander, M. G., Hitz, B. C., Tillakaratne, N. J. K., Kaufman, D. L., Wagner-McPherson, C. B., Evans, G. A., Tobin, A. J. : Two human glutamate decarboxylases, 65- kDa GAD and 67- kDa GAD, are each encoded by a single gene. Proc. Nat. Acad. Sci. 89: 2115-2119, 1992. 3. "Entrez Gene: GAD1 glutamate decarboxylase 1 (brain, 67 kDa)"

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Yang, Wang, He, Ding, Sun, Hua, Xi: "Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149004, (2016) (PubMed).
抗原

GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

别名

GAD1 (GAD1 产品)

背景

Synonyms: Glutamate decarboxylase 1,4.1.1.15,67 kDa glutamic acid decarboxylase,GAD-67,Glutamate decarboxylase 67 kDa isoform,GAD1,GAD, GAD67,
Tissue Specificity: Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .
Background: Glutamate decarboxylase 1 (brain, 67 kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Sequence Similarities: Belongs to the group II decarboxylase family.

分子量

67 kDa

基因ID

2571

UniProt

Q99259