Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) 抗体
Quick Overview for Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) 抗体 (ABIN3042370)
抗原
See all Crossover junction endonuclease EME1 (EME1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 520-561, C-Term
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原理
- Anti-EME1 Antibody Picoband®
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序列
- DKERQNLLAD IQVRRGEGVT STSRRIGPEL SRRIYLQMTT LQ
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-EME1 Antibody Picoband® (ABIN3042370). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human EME1, different from the related mouse sequence by five amino acids.
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亚型
- IgG
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anti-Crossover junction endonuclease EME1 (EME1) (AA 1-583) antibody
EME1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 1-583) antibodyEME1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (C-Term) antibodyEME1 适用: 人, 小鼠, Pig, 犬, 豚鼠, 兔, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (N-Term) antibodyEME1 适用: 人, 小鼠, Pig, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (AA 1-320) antibodyEME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Crossover junction endonuclease EME1 (EME1) (Internal Region) antibodyEME1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)". 2. Abraham, J., Lemmers, B., Hande, M. P., Moynahan, M. E., Chahwan, C., Ciccia, A., Essers, J., Hanada, K., Chahwan, R., Khaw, A. K., McPherson, P., Shehabeldin, A., Laister, R., Arrowsmith, C., Kanaar, R., West, S. C., Jasin, M., Hakem, R. Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells. EMBO J. 22: 6137-6147, 2003. 3. Ciccia A, Constantinou A, West SC (Jun 2003). "Identification and characterization of the human mus81-eme1 endonuclease". J Biol Chem 278 (27): 25172-8. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Crossover junction endonuclease EME1 (EME1)
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别名
- EME1
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背景
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Synonyms: Crossover junction endonuclease EME1,3.1.22.-,MMS4 homolog,hMMS4,EME1,MMS4,
Tissue Specificity: Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression. .
Background: Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene. It is mapped to 17q21.33. This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. Also, this protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.
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分子量
- 62 kDa
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基因ID
- 146956
抗原
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