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Villin 1 抗体 (C-Term)

This 兔 多克隆 anti-Villin 1 antibody specifically detects Villin 1 in IHC, WB, FACS 和 IF. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN3042343
发货至: 中国
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Quick Overview for Villin 1 抗体 (C-Term) (ABIN3042343)

抗原

See all Villin 1 (VIL1) 抗体
Villin 1 (VIL1)

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

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多克隆

标记

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This Villin 1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
  • 抗原表位

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    AA 770-799, C-Term

    原理

    Anti-Villin/VIL1 Antibody

    序列

    EQLVNKPVEE LPEGVDPSRK EEHLSIEDFT

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-Villin/VIL1 Antibody (ABIN3042343). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human Villin, different from the related mouse sequence by three amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    Villin 1 (VIL1)

    别名

    VIL1

    背景

    Synonyms: Villin-1,VIL1,VIL,

    Tissue Specificity: Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level). .

    Background: Villin is known as VIL1. This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed, they result from utilization of alternate poly-adenylation signals present in the terminal exon. In vertebrates, the villin proteins help to support the microfilaments of the microvilli of the brush border. It may play a role in cell plasticity through F-actin severing.

    Sequence Similarities: Belongs to the villin/gelsolin family.

    分子量

    93 kDa

    基因ID

    7429

    UniProt

    P09327

    途径

    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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