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UCHL1 抗体 (C-Term)

Cited in 1 publication. This anti-UCHL1 antibody is a 兔 多克隆 antibody detecting UCHL1 in WB, IHC, IF, FACS 和 ICC. Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN3042339
发货至: 中国
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Quick Overview for UCHL1 抗体 (C-Term) (ABIN3042339)

抗原

See all UCHL1 抗体
UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

适用

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人, 大鼠, 小鼠

宿主

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克隆类型

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多克隆

标记

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This UCHL1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 120-153, C-Term

    原理

    Anti-PGP9.5/UCHL1 Antibody

    序列

    ETEKMSPEDR AKCFEKNEAI QAAHDAVAQE GQCR

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-PGP9.5/UCHL1 Antibody (ABIN3042339). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Han, Chen, Huang, Zhu: "Nude mice multi-drug resistance model of orthotopic transplantation of liver neoplasm and Tc-99m MIBI SPECT on p-glycoprotein." in: World journal of gastroenterology, Vol. 11, Issue 22, pp. 3335-8, (2005) (PubMed).

  • 抗原

    UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

    别名

    UCHL1

    背景

    Synonyms: Ubiquitin carboxyl-terminal hydrolase isozyme L1,UCH-L1,3.4.19.12,6.-.-.-,Neuron cytoplasmic protein 9.5,PGP 9.5,PGP9.5,Ubiquitin thioesterase L1,UCHL1,

    Tissue Specificity: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. .

    Background: UCH-L1, also known as PGP9.5, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is abundantly present in all neurons (accounts for 1-2 % of total brain protein), expressed specifically in neurons and testis/ovary. The catalytic triad of UCH-L1 contains a cysteine at position 90, an aspartate at position 176, and a histidine at position 161 that are responsible for its hydrolase activity.

    分子量

    25 kDa

    基因ID

    7345

    UniProt

    P09936

    途径

    Feeding Behaviour
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