Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (fixed cells) (IF/ICC)
特异性
The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".
纯化方法
Purified from ascites by precipitation methods and size-exclusion chromatography
免疫原
Freshly ejaculated Human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.