MHC Class I H-2Ld 抗体 (Biotin)
The Biotin-conjugated 小鼠 单克隆 anti-MHC Class I H-2Ld antibody (Clone 30-5-7S) (ABIN303513) specifically detects MHC Class I H-2Ld in FACS.
The antibody is reactive with 小鼠 samples.
产品编号 ABIN303513
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class I H-2Ld 抗体 (Biotin) (ABIN303513)
抗原
MHC Class I H-2Ld
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS)
克隆位点
30-5-7S
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Protein G Chromatography.
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亚型
- IgG2a
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应用备注
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Flow Cytometry (See Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class I H-2Ld
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背景
- The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.Synonyms: H-2 class I histocompatibility antigen, H2-L, L-D alpha chain
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基因ID
- 10090
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UniProt
- P01897
抗原
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