MHC Class I H-2Ld 抗体 (Biotin)
适用: 小鼠
FACS
宿主: 小鼠
Monoclonal
30-5-7S
Biotin
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- 抗原
- MHC Class I H-2Ld
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适用
- 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- Flow Cytometry (FACS)
- 交叉反应 （详细）
- Species reactivity (tested):Mouse.
- 纯化方法
- Protein G Chromatography.
- 克隆位点
- 30-5-7S
- 亚型
- IgG2a
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- 应用备注
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Flow Cytometry (See Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium.
- 限制
- 仅限研究用
-
- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class I H-2Ld
- 背景
- The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.Synonyms: H-2 class I histocompatibility antigen, H2-L, L-D alpha chain
- 基因ID
- 10090
- UniProt
- P01897
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