Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
序列
DRENTTGSDN TDTEGS
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Immunoaffinity Chromatography.
免疫原
Synthetic peptide - KLH conjugated corresponding to Amino Acids 521-536 of Human CARD9. The sequence is different from that of Rat origin by two Amino Acids.
Western Blot (0.5-1 μg/mL). Immunocytochemistry. Immunohistochemistry on Paraffin Sections (10 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.02 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer. Dilute only prior to immediate use.
抗原
CARD9
(Caspase Recruitment Domain Family, Member 9 (CARD9))
Gm782 antibody, card9 antibody, CANDF2 antibody, hCARD9 antibody, caspase recruitment domain family, member 9 antibody, caspase recruitment domain family member 9 antibody, Card9 antibody, CARD9 antibody, card9 antibody
背景
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kB. CARD9 is an upstream activator of BCL-10 and NF-kB signaling.Synonyms: Caspase recruitment domain-containing protein 9