NOX5 抗体 (C-Term)
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Quick Overview for NOX5 抗体 (C-Term) (ABIN3031936)
抗原
See all NOX5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the C-terminus of human NADPH Oxidase 5 (KVLKGHCEKFGFRFFQENF) was used as the immunogen for this NOX5 antibody.
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亚型
- IgG
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the NOX5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the NOX5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- NOX5 (NADPH Oxidase 5 (NOX5))
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别名
- NOX5
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背景
- NADPH Oxidase 5, also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. It is a NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.
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UniProt
- Q96PH1
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途径
- Proton Transport
抗原
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