MPG 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for MPG 抗体 (C-Term) (ABIN3031925)
抗原
See all MPG 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
纯化方法
- Antigen affinity
-
免疫原
- An amino acid sequence from the C-terminus of human MPG (NKSFDQRDLAQDEAVW) was used as the immunogen for this MPG antibody.
-
亚型
- IgG
-
-
-
-
应用备注
- The stated application concentrations are suggested starting amounts. Titration of the MPG antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
-
限制
- 仅限研究用
-
-
-
缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
-
储存条件
- -20 °C
-
储存方法
- After reconstitution, the MPG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
-
-
- MPG (N-Methylpurine-DNA Glycosylase (MPG))
-
别名
- MPG
-
背景
- N-Methylpurine-DNA glycosylase is encoded by the MPG gene, mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. The gene was expressed in all cell lines and tissues examined, but was found at particularly high levels in a colon adenocarcinoma cell line (HT29). The completely characterized human gene was found to span 7 to 8 kb of genomic DNA and to be localized 75 kb upstream of the embryonic zeta-globin gene. To assess the contribution of MPG to the repair of several mutagenic lesions in vivo, Hang et al.(1997) biochemically analyzed cell-free extracts of tissues from mice with a targeted deletion of the gene. Following treatment with DNA-methylating agents, increased persistence of 7-methylguanine (meG) was found in liver sections of MPG knockout mice in comparison with wildtype mice, demonstrating an in vivo phenotype for the MPG-null animals.
-
UniProt
- P29372
-
途径
- DNA Damage Repair
抗原
-