HSP70 抗体 (AA 577-596)
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Quick Overview for HSP70 抗体 (AA 577-596) (ABIN3031162)
抗原
See all HSP70 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 577-596
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纯化方法
- Antigen affinity
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免疫原
- Amino acids 577-596 (VISWLDANTLAEKDEFEHKR-human) were used as the immunogen for this HSP70 antibody.
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亚型
- IgG
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应用备注
- The stated application concentrations are suggested starting points. Titration of the HSP70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the HSP70 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- HSP70 (Heat Shock Protein 70 (HSP70))
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别名
- HSP70
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背景
- HSPA1 (heat shock 70 kDa protein 1A) also known as HSPA1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. Shimizu et al.(1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of HSP72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.
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UniProt
- P08107
抗原
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