MYL9 抗体 (AA 12-27)
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北京 101111
Quick Overview for MYL9 抗体 (AA 12-27) (ABIN303105)
抗原
See all MYL9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 12-27
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特异性
- Amino acids 12-27 of Human Myosin light chain protein.
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交叉反应 （详细）
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Species reactivity (expected):Avian, Mammal, Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Synthetic peptide - KLH conjugated.
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anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (AA 1-100) antibody
MYL9 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (pSer20) antibodyMYL9 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (N-Term) antibodyMYL9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (pSer19), (pThr18) antibodyMYL9 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (pThr19) antibodyMYL9 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (AA 2-172) antibodyMYL9 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) antibodyMYL9 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB565 unconjugated Recombinant Antibody
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (pTyr118) antibodyMYL9 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (pSer15) antibodyMYL9 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myosin Regulatory Light Polypeptide 9 (MYL9) (pSer19) antibodyMYL9 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA (1/5000-1/20000). Western Blot (1/500-1/2000). Immunoprecipitation. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by Alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.71 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MYL9 (Myosin Regulatory Light Polypeptide 9 (MYL9))
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别名
- MYL9
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背景
- Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, Mouse and Rat have almost identical sequences.Synonyms: LC20, MLC-2C, MLC2, MRLC1, MYRL2, Myosin RLC, Myosin regulatory light chain 2, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, smooth muscle
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基因ID
- 10398
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NCBI登录号
- NP_006088
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UniProt
- P24844
抗原
-
