CAPN1 抗体 (Middle Region)
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Quick Overview for CAPN1 抗体 (Middle Region) (ABIN3030267)
抗原
See all CAPN1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the middle region of human CAPNL1 (EWNNVDPYERDQLRV) was used as the immunogen for this Calpain 1 antibody.
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亚型
- IgG
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anti-Calpain 1 (CAPN1) (AA 625-713) antibody
CAPN1 适用: 人, 小鼠, 大鼠 WB, FACS, IHC (p), ELISA, IF (cc), IF (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) antibodyCAPN1 适用: 人 WB, ICC, IF, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) antibodyCAPN1 适用: 人, 小鼠, 大鼠, Cow WB, IHC, IHC (p), ELISA, IP 宿主: 小鼠 Monoclonal 15C10 unconjugated
anti-Calpain 1 (CAPN1) antibodyKD Validated CAPN1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3290 unconjugated Recombinant Antibody
anti-Calpain 1 (CAPN1) (AA 468-481) antibodyCAPN1 适用: 人, Cow, Pig, 山羊, 兔, 豚鼠, 猴, 非洲爪蟾 WB, IHC, IHC (p), ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) (AA 515-526) antibodyCAPN1 适用: 人 WB, IHC, IHC (p), ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) (AA 300-349) antibodyCAPN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 山羊, 兔, 绵羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Calpain 1 (CAPN1) (AA 185-234) antibodyCAPN1 适用: 人, Cow, Pig, 山羊, 兔, 绵羊, 豚鼠, 猴, 非洲爪蟾, Bat, 小鸡, 犬, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the Calpain 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the Calpain 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- CAPN1 (Calpain 1 (CAPN1))
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别名
- Calpain 1
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背景
- Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, CAPN1 (mu-calpain) and CAPN2 (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 82 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of Calpain1/2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CAPNL1 to chromosome 11.
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UniProt
- P07384
抗原
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