Alkaline Phosphatase 抗体 (N-Term)
Quick Overview for Alkaline Phosphatase 抗体 (N-Term) (ABIN3029905)
抗原
See all Alkaline Phosphatase (ALP) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the N-terminus of rat ALPL (FVPEKEKDPSYWRQQ) was used as the immunogen for this tissue-nonspecific Alkaline Phosphatase antibody.
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亚型
- IgG
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anti-Alkaline Phosphatase (ALP) (AA 56-83) antibody
ALP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 282-309) antibodyALP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人, Cow IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase (ALP) (AA 21-35) antibodyALP 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人, Bacteria WB, ELISA 宿主: 小鼠 Monoclonal BGN-03-662 unconjugated
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应用备注
- The stated application concentrations are suggested starting points. Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the Alkaline Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- Alkaline Phosphatase (ALP)
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别名
- Alkaline Phosphatase
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背景
- Alkaline phosphatase removes phosphate groups from the 5-prime end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non-tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. ALPL is the alkaline phosphatase of skin fibroblasts, the tissue-nonspecific type, and that it is active toward millimolar concentrations of the putative natural substrates phosphoethanolamine(PEA) and pyridoxal-5-prime-phosphate(PLP). ALPL gene exists in single copy in the haploid genome and is composed of 12 exons distributed over more than 50 kb. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
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UniProt
- P08289
抗原
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