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Vimentin 抗体 (full length)

Name: Vimentin 抗体 (full length)
SKU: ABIN3026904
Availability: OutOfStock

The 小鼠单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from 人. It has been validated for WB 和 IHC (p).

Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with anti-Vimentin antibody (VM1170). (Vimentin 抗体 (full length))

IHC (fp)

Formalin-fixed, paraffin-embedded human melanoma stained with anti-Vimentin antibody (VM1170). (Vimentin 抗体 (full length))

IHC (fp)

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with anti-Vimentin antibody. (Vimentin 抗体 (full length))

IHC (fp)

Formalin-fixed, paraffin-embedded human uterus stained with anti-Vimentin antibody. (Vimentin 抗体 (full length))

IHC (fp)

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with anti-Vimentin antibody. (Vimentin 抗体 (full length))

IHC (fp)

5 images

产品编号 ABIN3026904

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (full length) (ABIN3026904)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

Carrier-free

克隆位点

VM1170

Discover our top product VIM Primary Antibody

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full length

原理

Anti-Vimentin Antibody (azide and preservative free)

无交叉反应

小鼠, 大鼠

产品特性

This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

纯化方法

Protein G affinity chromatography

免疫原

Recombinant full-length human protein was used as the immunogen for the anti-Vimentin antibody.

亚型

IgG1, kappa
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

Optimal dilution of the anti-Vimentin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1 mg/mL in 1X PBS, BSA free, sodium azide free

储存液

Azide free

储存条件

4 °C,-20 °C

储存方法

Store the anti-Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
抗原

Vimentin (VIM)

别名

Vimentin

背景

This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

UniProt

P08670

途径

Caspase Cascade in Apoptosis