lambda 抗体
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北京 101111
Quick Overview for lambda 抗体 (ABIN3026856)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified human IgG was used as the immunogen for this anti-Lambda antibody.
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亚型
- IgG2a kappa
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anti-lambda antibody (FITC)
适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal FITC
anti-lambda antibody (Biotin)适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal Biotin
anti-lambda antibody (Alkaline Phosphatase (AP))适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
anti-lambda antibody (HRP)适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal HRP F(ab')2 fragment
anti-lambda antibody (PE)适用: 人 FACS, ELISA, FLISA 宿主: 山羊 Polyclonal PE
anti-lambda antibody适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal LAM03 unconjugated
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应用备注
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The optimal dilution of the anti-Lambda antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Lambda antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- lambda
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背景
- This mAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
抗原
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