Nucleoli Marker 抗体
The 小鼠 单克隆 anti-Nucleoli Marker antibody is suitable to detect Nucleoli Marker in samples from 人. It has been validated for IF, FACS, ICC 和 IHC (p).
产品编号 ABIN3026802
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Nucleoli Marker 抗体 (ABIN3026802)
抗原
Nucleoli Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
NM95
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产品特性
- This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Nuclei of myeloid leukemia biopsy cells were used as the immunogen.
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亚型
- IgG1 kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed): 0.5-1 μg/mL for 30 min at RT,IHC (Formalin-paraffin): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Nucleoli marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Nucleoli Marker
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背景
- This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
抗原
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