Cytokeratin HMW (KRT HMW) 抗体
This 小鼠 单克隆 antibody specifically detects Cytokeratin HMW in IF, WB, FACS 和 IHC (p). It exhibits reactivity toward 人, 大鼠 和 小鼠.
1 image
产品编号 ABIN3026726
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Cytokeratin HMW (KRT HMW) 抗体 (ABIN3026726)
抗原
See all Cytokeratin HMW (KRT HMW) 抗体
Cytokeratin HMW (KRT HMW)
适用
人, 大鼠, 小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
质量等级
Carrier-free
克隆位点
34BE12
-
-
原理
- HMW Cytokeratin Antibody / Basic / Type II (azide and preservative free)
-
产品特性
- This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
-
亚型
- IgG1, kappa
-
-
-
-
应用备注
-
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the HMW Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
-
-
- Cytokeratin HMW (KRT HMW)
-
别名
- HMW Cytokeratin
-
背景
- This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
抗原
-
You are here:
