Cyclin B1 抗体
Quick Overview for Cyclin B1 抗体 (ABIN3026645)
抗原
See all Cyclin B1 (CCNB1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody recognizes a protein of 55-62 kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Hamster protein was used as the immunogen for this Cyclin B1 antibody.
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亚型
- IgG1 kappa
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anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyCCNB1 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal CCNB1-1098 unconjugated
anti-Cyclin B1 (CCNB1) (C-Term) antibodyCCNB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 271-433) antibodyCCNB1 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-433) antibodyCCNB1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (AA 1-90) antibodyCCNB1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1C8 unconjugated
anti-Cyclin B1 (CCNB1) (pSer126) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) (Internal Region) antibodyCCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin B1 (CCNB1) antibodyKO Validated CCNB1 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IP: 1-2 μg/500 μg protein--precipitates active Cyclin B1 complexes
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Cyclin B1 (CCNB1)
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别名
- Cyclin B1
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背景
- This antibody recognizes a protein of 55-62 kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
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基因ID
- 891
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途径
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
抗原
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