GFAP 抗体
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北京 101111
Quick Overview for GFAP 抗体 (ABIN3026568)
抗原
See all GFAP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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纯化方法
- Protein G affinity chromatography
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免疫原
- GFAP isolated from pig spinal cord was used as the immunogen.
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亚型
- IgG1
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anti-Glial Fibrillary Acidic Protein (GFAP) (AA 51-150) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyGFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibodyGFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyVerified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibodyGFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GFAP antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store the GFAP antibody at 2-8°C.
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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别名
- GFAP
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背景
- This antibody recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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基因ID
- 2670
抗原
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