MLANA 抗体
Quick Overview for MLANA 抗体 (ABIN3026558)
抗原
See all MLANA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human Melan-A protein was used as the immunogen for this antibody.
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亚型
- IgG2b kappa
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anti-Melan A (MLANA) antibody
MLANA 适用: 人, 小鼠, 大鼠 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1761R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal M2-9E3 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 马 IHC, ISt, StM 宿主: 小鼠 Monoclonal MLANA-1761R unconjugated
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) (AA 1-118) antibodyMLANA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal A103 unconjugated
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 兔 Monoclonal MLANA-1409R unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人, 小鼠, 大鼠, 犬 IHC, ISt, StM 宿主: 小鼠 Monoclonal rMLANA-788 unconjugated Recombinant Antibody
anti-Melan A (MLANA) antibodyMLANA 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-901M- unconjugated Recombinant Antibody
anti-Melan A (MLANA) (AA 48-118) antibodyMLANA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F3B7 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- MLANA (Melan A (MLANA))
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别名
- Melan-A
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背景
- This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
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基因ID
- 2315
抗原
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