Ku70 + Ku80 抗体
This anti-Ku70 + Ku80 antibody is a 小鼠 单克隆 antibody detecting Ku70 + Ku80 in FACS, IF 和 ICC. Suitable for 人 和 灵长类.
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Quick Overview for Ku70 + Ku80 抗体 (ABIN3026515)
抗原
Ku70 + Ku80 (KU80)
(CG18801 Gene Product From Transcript CG18801-RA (KU80))
适用
人, 灵长类
宿主
小鼠
克隆类型
单克隆
标记
This Ku70 + Ku80 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
克隆位点
KU729
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无交叉反应
- 小鼠, 大鼠, 小鸡
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产品特性
- This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
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纯化方法
- Protein G purified monoclonal antibody
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免疫原
- Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
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亚型
- IgG1 kappa
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anti-CG18801 Gene Product From Transcript CG18801-RA (KU80) antibody (PE)
KU80 适用: 人, 灵长类 FACS, IF 宿主: 小鼠 Monoclonal KU729 PE
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应用备注
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.\. Flow cytometry: 0.5-1 μg/10e6 cells,IF: 0.5-1.0 μg/mL,Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 μg/mL for 30 minutes at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Ku70 + Ku80 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))
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别名
- Ku70 + Ku80
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背景
- This antibody recognizes a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
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基因ID
- 2547
抗原
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