CDK1 抗体
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北京 101111
Quick Overview for CDK1 抗体 (ABIN3026474)
抗原
See all CDK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 小鼠, 大鼠, 非洲爪蟾, Fruit Fly (Drosophila melanogaster), Schizosaccharomyces pombe, Yeast (Saccharomyces cerevisiae)
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产品特性
- This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
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纯化方法
- Protein G purified antibody
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免疫原
- Purified recombinant human Cdk1 / p34cdc2 was used as the immunogen for this Cdk1 antibody.
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亚型
- IgG2a kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdk1 antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE) only: 1-2 μg/mL (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cdk1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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别名
- Cdk1
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背景
- This antibody recognizes a 34 kDa protein, which is identified as cyclin dependent kinase 1 (Cdk1) or p34cdc2 protein kinase. CDK1 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated CDK1 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
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基因ID
- 983
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
抗原
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