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CD47 抗体

The 小鼠 单克隆 anti-CD47 antibody (Clone IAP-964) (ABIN3026437) specifically detects CD47 in FACS, IF 和 IHC (p). The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN3026437
发货至: 中国
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Quick Overview for CD47 抗体 (ABIN3026437)

抗原

See all CD47 抗体
CD47

适用

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人, 小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD47 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

IAP-964
  • 产品特性

    This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

    纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for the CD47 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the CD47 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the CD47 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    CD47

    别名

    CD47 (IAP)

    背景

    This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50  kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
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