SUMO2/3 抗体
Quick Overview for SUMO2/3 抗体 (ABIN3026430)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This antibody reacts with both SUMO2 and SUMO3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursors that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO1/2/3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO1 utilizes Ubc9 for conjugation to several targets, which include MDM2, p53, PML and RanGap1. SUMO2/3 contribute to a greater percentage of protein modification than does SUMO1 and they can form polymeric chains. In addition, SUMO3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.
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纯化方法
- Protein G purified
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免疫原
- Recombinant human SUMO2 protein was used as the immunogen for this SUMO2/3 antibody.
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亚型
- IgG1 kappa
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anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (full length) antibody
SUMO2/3 适用: 人, 大鼠, 小鼠 WB, IF, ELISA, IHC (fro) 宿主: 大鼠 Monoclonal 3H12 unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 49-81) antibodySUMO2/3 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 53-84), (C-Term) antibodySUMO2/3 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15398 unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 53-84) antibodySUMO2/3 适用: 人 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (cleaved), (Gly93) antibodySUMO2/3 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Small Ubiquitin Related Modifier 2/3 (SUMO2/3) (AA 38-64) antibodySUMO2/3 适用: 人 WB 宿主: 兔 Polyclonal RB3377 unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the SUMO2/3 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the SUMO2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))
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别名
- SUMO2/3
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背景
- This antibody reacts with both SUMO2 and SUMO3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursors that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO1/2/3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO1 utilizes Ubc9 for conjugation to several targets, which include MDM2, p53, PML and RanGap1. SUMO2/3 contribute to a greater percentage of protein modification than does SUMO1 and they can form polymeric chains. In addition, SUMO3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer's disease.
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基因ID
- 6613
抗原
-
