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- 抗原 See all HCG beta 抗体
- HCG beta
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HCG beta antibody is un-conjugated
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应用范围
- ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody reacts with a protein of 22 kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant protein was used as the immunogen for this HCG-beta antibody.
- 克隆位点
- HCGb-54
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product HCG beta Primary Antibody
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anti-HCG beta (AA 81-165) antibody
适用: 人 ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-HCG beta antibody适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-HCG beta (C-Term) antibody适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HCG beta (AA 101-150) antibody适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.
1. Clone HCGb/54 (recommended detect) will pair with clones < a href=../tds/hcg-beta-antibody-hcgb211-v2374>HCGb/211 (recommended capture) and < a href=../tds/hcg-beta-antibody-hcgb459-v2373>HCGb/459 by ELISA.
2. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: use BSA-free format (1),IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (2),Prediluted format : incubate for 30 min at RT (3) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the HCG-beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- HCG beta
- 别名
- HCG-beta (HCG beta 产品)
- 背景
- This antibody reacts with a protein of 22 kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
- 基因ID
- 1082
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