Melanoma Cell Marker 抗体
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北京 101111
Quick Overview for Melanoma Cell Marker 抗体 (ABIN3026335)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Melanoma Cell Marker Antibody Cocktail
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产品特性
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant MART-1 protein (M2-7C10 & M2-9E3), recombinant tyrosinase protein (T311) and extract of pigmented melanoma metastases from lymph nodes (HMB45) were used as the immunogens for this Melanoma Cell Marker antibody cocktail.
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亚型
- IgG1, IgG2a, IgG2b
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anti-Melanoma Cell Marker antibody
Carrier-free 适用: 人 IHC (p), WB 宿主: 小鼠 Monoclonal M2-7C10-M2-9E3-HMB45-T311 unconjugated
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应用备注
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The optimal dilution of the Melanoma Cell Marker antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Melanoma Cell Marker antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Melanoma Cell Marker
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背景
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
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UniProt
- Q16655
抗原
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