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CD68 抗体

CD68 适用: 人, 小鼠, 大鼠, 猴, Cat FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal KP1-C68-684 unconjugated
产品编号 ABIN3026325
发货至: 中国
  • 抗原 See all CD68 抗体
    CD68 (CD68 Molecule (CD68))
    适用
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    • 61
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    人, 小鼠, 大鼠, 猴, Cat
    宿主
    • 83
    • 73
    • 11
    • 1
    小鼠
    克隆类型
    • 99
    • 65
    单克隆
    标记
    • 82
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    This CD68 antibody is un-conjugated
    应用范围
    • 74
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    产品特性
    This antibody cocktail recognizes a glycoprotein of 110 kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
    纯化方法
    Protein G affinity chromatography
    免疫原
    The subcellular fraction of human alveolar macrophages was used as the immunogen for this CD68 antibody.
    克隆位点
    KP1-C68-684
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD68 Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD68 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: use antibody at 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    Store the CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    CD68 (CD68 Molecule (CD68))
    别名
    CD68 (CD68 产品)
    别名
    Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody
    背景
    This antibody cocktail recognizes a glycoprotein of 110  kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
    基因ID
    968
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