PAX7 抗体 (AA 300-600)
Quick Overview for PAX7 抗体 (AA 300-600) (ABIN3026252)
抗原
See all PAX7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 300-600
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纯化方法
- Protein G affinity chromatography
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免疫原
- A recombinant fragment (aa300-600) was used as the immunogen for the PAX7 antibody.
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亚型
- IgG1 kappa
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应用备注
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Optimal dilution of the PAX7 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the PAX7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- PAX7 (Paired Box 7 (PAX7))
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别名
- PAX7
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背景
- The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
抗原
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