PLA2G3 抗体 (Internal Region)
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Quick Overview for PLA2G3 抗体 (Internal Region) (ABIN302624)
抗原
See all PLA2G3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody detects Group III Secreted Phospholipase A2 (GIII-SPLA2) (PLA2G3) at internal domain.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- Synthetic peptide - KLH conjugated, internal domain of human
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应用备注
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Immunohistochemistry on paraffin sections (2.5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline containing 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer.
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- PLA2G3 (Phospholipase A2, Group III (PLA2G3))
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别名
- PLA2G3 / sPLA2-III
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背景
- Human group III secreted phospholipase A2 belongs to a superfamily of intracellular and secreted enzymes that catalyze the hydrolysis of glycerophospholipids at the sn-2 position to release fatty acids and lysophospholipids. Group III PLA2 enzymes were originally identified in invertebrates such as bees and scorpions. Human group III sPLA2 has been shown to be a calcium dependent enzyme. It consists of a central group III sPLA2 domain flanked by unique N- and C-terminal domains that are proteolytically removed in most tissues. The sPLA2 domain is sufficient for catalytic activity. SPLA2-III is preferentially expressed in the microvascular endothelium of tissues with inflammation, ischemic injury and cancer.Synonyms: Group 3 secretory phospholipase A2, Group III secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase GIII
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基因ID
- 50487
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NCBI登录号
- NP_056530
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UniProt
- Q9NZ20
抗原
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