Blood Group H 抗体
The 小鼠 单克隆 anti-Blood Group H antibody (Clone 19-OLE) (ABIN3026086) specifically detects Blood Group H in IHC (p) 和 IF.
The antibody is reactive with 人 samples.
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产品编号 ABIN3026086
发货至:
中国
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中国
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Quick Overview for Blood Group H 抗体 (ABIN3026086)
抗原
Blood Group H
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
克隆位点
19-OLE
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纯化方法
- PEG precipitation
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免疫原
- Mucinous colonic adenocarcinoma was used as the immunogen for the ABO antibody.
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亚型
- IgM kappa
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anti-Blood Group H antibody
适用: 人 IHC (p), IF 宿主: 小鼠 Monoclonal 19-OLE unconjugated
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应用备注
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Optimal dilution of the ABO antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Blood Group H
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别名
- ABO (Blood Group Antigen H Type 2)
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背景
- Recognizes the blood group H type 2 antigens, trisaccharide Fuc(a1-2)Gal(b1-4)GlcNAc(b1) of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.
抗原
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