S100A8/A9 Complex (Calprotectin) 抗体
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Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (ABIN3026084)
抗原
See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- MRP14 Antibody / S100A9 (azide and preservative free)
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纯化方法
- Protein G affinity chromatography
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免疫原
- An affinity purified monocyte membrane preparation was used as the immunogen for the MRP14 antibody.
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亚型
- IgG1, kappa
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应用备注
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Optimal dilution of the MRP14 antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the MRP14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
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别名
- MRP8 + MPR14
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背景
- Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
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UniProt
- P06702
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途径
- S100 Proteins
抗原
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