S100A8/A9 Complex (Calprotectin) 抗体
Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (ABIN3026084)
抗原
See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- An affinity purified monocyte membrane preparation was used as the immunogen for the MRP8 + MPR14 antibody.
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亚型
- IgG1 kappa
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anti-Calprotectin (S100A8/A9) antibody
S100A8/A9 适用: 人, Pig, Cow, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠, 马, Baboon, 山羊 IHC, StM 宿主: 小鼠 Monoclonal rMAC3781 unconjugated Recombinant Antibody
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人, Pig, Cow, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠, 马, Baboon, 山羊 IHC, StM 宿主: 兔 Monoclonal MAC3157R unconjugated Recombinant Antibody
anti-Calprotectin (S100A8/A9) (AA 1-114) antibodyS100A8/A9 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Calprotectin (S100A8/A9) antibodyAnimal-Free S100A8/A9 适用: 人 ELISA 宿主: 小鼠 Monoclonal AB467-1-A05 unconjugated Recombinant Antibody
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal 69-6-1 unconjugated
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应用备注
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Optimal dilution of the MRP8 + MPR14 antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the MRP8 + MPR14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
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别名
- MRP8 + MPR14
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背景
- Recognizes S100A8/MPR8 and S100A9/MRP14, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
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途径
- S100 Proteins
抗原
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