BCL6 抗体
Quick Overview for BCL6 抗体 (ABIN3025978)
抗原
See all BCL6 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for the anti-Bcl6 antibody.
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亚型
- IgG1 kappa
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anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 501-600) antibody
BCL6 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (Internal Region) antibodyBCL6 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 256-389) antibodyBCL6 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal BCL6-1527 unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (Internal Region) antibodyBCL6 适用: 人, 小鼠 WB, IHC (p), IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 147-276) antibodyBCL6 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2G1B6 unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (Internal Region) antibodyBCL6 适用: 人, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 519-547) antibodyBCL6 适用: 人, 小鼠 WB, IHC, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 256-389) antibodyBCL6 适用: 人 WB 宿主: 小鼠 Monoclonal BCL6-1475 unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (AA 256-389) antibodyBCL6 适用: 人 IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal BCL6-1982 unconjugated
anti-B-Cell CLL/lymphoma 6 (BCL6) (pSer333) antibodyBCL6 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the anti-Bcl6 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the anti-Bcl6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- BCL6 (B-Cell CLL/lymphoma 6 (BCL6))
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别名
- Bcl6
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背景
- Recognizes a protein of 95 kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
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途径
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
抗原
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