TTF1 抗体
Quick Overview for TTF1 抗体 (ABIN3025853)
抗原
See all TTF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant protein was used as the immunogen for the TTF1 antibody cocktail.
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亚型
- IgG
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anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 201-300) antibody
TTF1 适用: 人, 大鼠, 小鼠 WB, FACS, ICC, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 1-150) antibodyTTF1 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F4D8 unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (Internal Region) antibodyTTF1 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 51-100) antibodyTTF1 适用: 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 412-461) antibodyTTF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 97-110), (N-Term) antibodyTTF1 适用: 人 IHC (p) 宿主: 兔 Monoclonal G21-G unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (AA 1-204) antibodyTTF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Termination Factor, RNA Polymerase I (TTF1) (N-Term) antibodyTTF1 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the TTF1 antibody cocktail should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the TTF1 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
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别名
- TTF1
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背景
- Recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
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途径
- Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
抗原
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