Cytokeratin 19 抗体
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北京 101111
Quick Overview for Cytokeratin 19 抗体 (ABIN3025844)
抗原
See all Cytokeratin 19 (KRT19) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- CK19 Antibody / Cytokeratin 19 (azide and preservative free)
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant human Cytokeratin 19 protein was used as the immunogen for the CK19 antibody.
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亚型
- IgG1, kappa
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应用备注
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Optimal dilution of the CK19 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the CK19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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别名
- CK19
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背景
- Recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
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UniProt
- P08727
抗原
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